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Image Search Results
Journal: Advanced Materials (Deerfield Beach, Fla.)
Article Title: Unlocking Intracellular Protein Delivery by Harnessing Polymersomes Synthesized at Microliter Volumes using Photo‐PISA
doi: 10.1002/adma.202408000
Figure Lengend Snippet: In vitro activation of BMDCs and antigen cross‐presentation mediated by OVA‐loaded pH‐responsive PSomes. A) Schematic illustration of OVA‐loaded pH‐responsive PSomes enhancing maturation and antigen cross‐presentation in dendritic cells. Characterization of surface markers B) CD80, C) CD86, D) MHC class II, and E) SIINKEFL (OVA (257‐264)) bound to MHC class I by immunoflow cytometry analysis under various treatments in CD11c + cells. Cells were stained with antibodies against CD11c as the DC marker, CD80, CD86, and MHC class II as DC maturation markers as well as SIINKEFL‐H2K b to measure OVA antigen cross‐presentation. Data presented as mean ± SD ( n = 3, technical replicates). Statistical significance was determined using one‐way ANOVA with Dunnett's multiple comparisons test. **** p < 0.0001; ns not significant.
Article Snippet: A useful tool in this regard to study the loading of macromolecules on a single‐particle basis is
Techniques: In Vitro, Activation Assay, Cytometry, Staining, Marker